Investigative Toxicology Summer Intern
Celgene’s Investigative Toxicology group is interested in sponsoring a summer intern to focus on the development of in vitro assays to monitor the effects of prolonged interleukin-2 (IL-2) exposure on T cell polarization and regulatory T cell plasticity. If time permits, the summer intern may also explore the role of IL-2 in eosinophil degranulation.
The summer intern will culture human and cynomolgus monkey blood cells, including lymphocytes and granulocytes, perform RT-PCR to characterize expression of key transcription factors, measure cytokine secretion through electrochemiluminescence, and immunophenotype the cells via flow cytometry.
The resulting data will be analyzed and used to improve our understanding of IL-2 therapy and the pathogenesis of type 2 proinflammatory responses.
The intern will be required to record all experiments in an electronic laboratory notebook. The student will also present their work at weekly investigative toxicology group meetings and at an internal scientific poster fair at BMS towards the end of the internship.
Skills/Knowledge Required:
- Tissue Culture, some exposure to cell-based assays, and ability to analyze scientific data
- Records experimental details
- Maintains laboratory equipment, supplies and notebook
- Searches literature for targeted information
Summer Intern will gain:
- Advanced cell culture skills
- Expand their knowledge of mammalian immunology
- Enhanced laboratory skills and exposure to robust technology (flow cytometry, RT-PCR/Taqman, and Meso Scale Discovery assays)
- An understanding of Pharmaceutical industry laboratory practices and record keeping
- Experience working in a highly productive and collaborative laboratory
- Presentation and interpersonal skills